Haemophilus ducreyi Lipooligosaccharide Mutant Defective in Expression of β-1,4-Glucosyltransferase Is Virulent in Humans
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منابع مشابه
A superoxide dismutase C mutant of Haemophilus ducreyi is virulent in human volunteers.
Haemophilus ducreyi produces a periplasmic copper-zinc superoxide dismutase (Cu-Zn SOD), which is thought to protect the organism from exogenous reactive oxygen species generated by neutrophils during an inflammatory response. We had previously identified the gene, sodC, responsible for the production and secretion of Cu-Zn SOD and constructed an isogenic H. ducreyi strain with a mutation in th...
متن کاملUse of pyocin to select a Haemophilus ducreyi variant defective in lipooligosaccharide biosynthesis.
Haemophilus ducreyi, a cause of genital ulcer disease in developing countries, appears to facilitate the heterosexual transmission of the human immunodeficiency virus in Africa. Despite an increase in studies of this gram-negative human pathogen, little is known about the pathogenesis of chancroid. Our studies have shown that the lipooligosaccharides (LOS) of H. ducreyi may play an important ro...
متن کاملA Haemophilus ducreyi CpxR deletion mutant is virulent in human volunteers.
Haemophilus ducreyi 35000HP contains a homolog of the CpxRA 2-component signal transduction system, which controls the cell envelope stress response system in other gram-negative bacteria and regulates some important H. ducreyi virulence factors. A H. ducreyi cpxR mutant was compared with its parent for virulence in the human challenge model of experimental chancroid. The pustule formation rate...
متن کاملConstruction and Characterization of Haemophilus ducreyi Lipooligosaccharide (LOS) Mutants Defective in Expression of Heptosyltransferase III and b1,4-Glucosyltransferase: Identification of LOS Glycoforms Containing Lactosamine Repeats
MELANIE J. FILIATRAULT, BRADFORD W. GIBSON, BIRGIT SCHILLING, SHUHUA SUN, ROBERT S. MUNSON, JR., AND ANTHONY A. CAMPAGNARI* Department of Microbiology, Department of Medicine, Division of Infectious Diseases, and Center for Microbial Pathogenesis, University at Buffalo, Buffalo, New York 14214; Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143-044...
متن کاملInvolvement of the Haemophilus ducreyi gmhA gene product in lipooligosaccharide expression and virulence.
The lipooligosaccharide (LOS) present in the outer membrane of Haemophilus ducreyi is likely a virulence factor for this sexually transmitted pathogen. An open reading frame in H. ducreyi 35000 was found to encode a predicted protein that had 87% identity with the protein product of the gmhA (isn) gene of Haemophilus influenzae. In H. influenzae type b, inactivation of the gmhA gene caused the ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 2001
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.69.6.4180-4184.2001